script ii rt kit Search Results


onestep tb green � primescripttm rt qpcr kit ii  (TaKaRa)
96
TaKaRa onestep tb green � primescripttm rt qpcr kit ii
Onestep Tb Green � Primescripttm Rt Qpcr Kit Ii, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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superprep ii cell lysis kit toyobo  (Toyobo)
95
Toyobo superprep ii cell lysis kit toyobo
Superprep Ii Cell Lysis Kit Toyobo, supplied by Toyobo, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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hiscript ii onestep rt pcr kit  (Vazyme Biotech Co)
96
Vazyme Biotech Co hiscript ii onestep rt pcr kit
Hiscript Ii Onestep Rt Pcr Kit, supplied by Vazyme Biotech Co, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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p612 hiscript ii  (Vazyme Biotech Co)
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Vazyme Biotech Co p612 hiscript ii
P612 Hiscript Ii, supplied by Vazyme Biotech Co, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rt pcr kit  (PCR Biosystems Ltd)
94
PCR Biosystems Ltd rt pcr kit
(A) Schematic of the putative bb0605 operon, as well as the downstream bb0608 locus, mapped from the B. burgdorferi B31 type strain genome. The transposon integration site (+300, 5’ to 3’) in bb0605 is shown (triangle) as are the length, and location, of predicted non-coding regions. (B) Relative mRNA levels of target loci to validate B31-5A3/ bb0605 <t>mutant.</t> <t>RT-PCR</t> was performed on RNA isolated from both parental (B31-5A3, charcoal) and mutant (B31-5A3/ bb0605, light gray) strains using locus-specific primers and normalized relative to constitutively expressed flaB. Values are the mean + /- SD. n.d., not detected after 45 cycles. (C) Identifying changes in the peptidoglycan composition of B31-5A3/ bb0605 . Liquid chromatogram of mutanolysin treated PG isolated from B31-5A3 (top, in black) and B31-5A3/ bb0605 (bottom, in green). Each numbered peak corresponds to a muropeptide found in and , respectively. Peak 5 (shaded gray) corresponds to the muropeptide Glc N Ac-Mur N Ac-Ala-Glu-Orn-[Gly]-Ala-Ala). (D) MS (top) and MS/MS (bottom) of the Glc N Ac-Mur N Ac-Ala-Glu-Orn-[Gly]-Ala-Ala muropeptide. MS/MS fragmentation (inset) data confirms the pentapeptide(-Gly) structure.
Rt Pcr Kit, supplied by PCR Biosystems Ltd, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rt pcr kit - by Bioz Stars, 2026-05
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reverse transcription pcr rt pcr  (Jena Bioscience)
94
Jena Bioscience reverse transcription pcr rt pcr
(A) Schematic of the putative bb0605 operon, as well as the downstream bb0608 locus, mapped from the B. burgdorferi B31 type strain genome. The transposon integration site (+300, 5’ to 3’) in bb0605 is shown (triangle) as are the length, and location, of predicted non-coding regions. (B) Relative mRNA levels of target loci to validate B31-5A3/ bb0605 <t>mutant.</t> <t>RT-PCR</t> was performed on RNA isolated from both parental (B31-5A3, charcoal) and mutant (B31-5A3/ bb0605, light gray) strains using locus-specific primers and normalized relative to constitutively expressed flaB. Values are the mean + /- SD. n.d., not detected after 45 cycles. (C) Identifying changes in the peptidoglycan composition of B31-5A3/ bb0605 . Liquid chromatogram of mutanolysin treated PG isolated from B31-5A3 (top, in black) and B31-5A3/ bb0605 (bottom, in green). Each numbered peak corresponds to a muropeptide found in and , respectively. Peak 5 (shaded gray) corresponds to the muropeptide Glc N Ac-Mur N Ac-Ala-Glu-Orn-[Gly]-Ala-Ala). (D) MS (top) and MS/MS (bottom) of the Glc N Ac-Mur N Ac-Ala-Glu-Orn-[Gly]-Ala-Ala muropeptide. MS/MS fragmentation (inset) data confirms the pentapeptide(-Gly) structure.
Reverse Transcription Pcr Rt Pcr, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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dengue fever virus general-type real time rt–pcr kit  (Liferiver Bio Tech Corp)
90
Liferiver Bio Tech Corp dengue fever virus general-type real time rt–pcr kit
(A) Schematic of the putative bb0605 operon, as well as the downstream bb0608 locus, mapped from the B. burgdorferi B31 type strain genome. The transposon integration site (+300, 5’ to 3’) in bb0605 is shown (triangle) as are the length, and location, of predicted non-coding regions. (B) Relative mRNA levels of target loci to validate B31-5A3/ bb0605 <t>mutant.</t> <t>RT-PCR</t> was performed on RNA isolated from both parental (B31-5A3, charcoal) and mutant (B31-5A3/ bb0605, light gray) strains using locus-specific primers and normalized relative to constitutively expressed flaB. Values are the mean + /- SD. n.d., not detected after 45 cycles. (C) Identifying changes in the peptidoglycan composition of B31-5A3/ bb0605 . Liquid chromatogram of mutanolysin treated PG isolated from B31-5A3 (top, in black) and B31-5A3/ bb0605 (bottom, in green). Each numbered peak corresponds to a muropeptide found in and , respectively. Peak 5 (shaded gray) corresponds to the muropeptide Glc N Ac-Mur N Ac-Ala-Glu-Orn-[Gly]-Ala-Ala). (D) MS (top) and MS/MS (bottom) of the Glc N Ac-Mur N Ac-Ala-Glu-Orn-[Gly]-Ala-Ala muropeptide. MS/MS fragmentation (inset) data confirms the pentapeptide(-Gly) structure.
Dengue Fever Virus General Type Real Time Rt–Pcr Kit, supplied by Liferiver Bio Tech Corp, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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improm rt-pcr kit  (Promega)
90
Promega improm rt-pcr kit
(A) Schematic of the putative bb0605 operon, as well as the downstream bb0608 locus, mapped from the B. burgdorferi B31 type strain genome. The transposon integration site (+300, 5’ to 3’) in bb0605 is shown (triangle) as are the length, and location, of predicted non-coding regions. (B) Relative mRNA levels of target loci to validate B31-5A3/ bb0605 <t>mutant.</t> <t>RT-PCR</t> was performed on RNA isolated from both parental (B31-5A3, charcoal) and mutant (B31-5A3/ bb0605, light gray) strains using locus-specific primers and normalized relative to constitutively expressed flaB. Values are the mean + /- SD. n.d., not detected after 45 cycles. (C) Identifying changes in the peptidoglycan composition of B31-5A3/ bb0605 . Liquid chromatogram of mutanolysin treated PG isolated from B31-5A3 (top, in black) and B31-5A3/ bb0605 (bottom, in green). Each numbered peak corresponds to a muropeptide found in and , respectively. Peak 5 (shaded gray) corresponds to the muropeptide Glc N Ac-Mur N Ac-Ala-Glu-Orn-[Gly]-Ala-Ala). (D) MS (top) and MS/MS (bottom) of the Glc N Ac-Mur N Ac-Ala-Glu-Orn-[Gly]-Ala-Ala muropeptide. MS/MS fragmentation (inset) data confirms the pentapeptide(-Gly) structure.
Improm Rt Pcr Kit, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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suprimescript cdna synthesis kit  (Genet Bio Inc)
90
Genet Bio Inc suprimescript cdna synthesis kit
(A) Schematic of the putative bb0605 operon, as well as the downstream bb0608 locus, mapped from the B. burgdorferi B31 type strain genome. The transposon integration site (+300, 5’ to 3’) in bb0605 is shown (triangle) as are the length, and location, of predicted non-coding regions. (B) Relative mRNA levels of target loci to validate B31-5A3/ bb0605 <t>mutant.</t> <t>RT-PCR</t> was performed on RNA isolated from both parental (B31-5A3, charcoal) and mutant (B31-5A3/ bb0605, light gray) strains using locus-specific primers and normalized relative to constitutively expressed flaB. Values are the mean + /- SD. n.d., not detected after 45 cycles. (C) Identifying changes in the peptidoglycan composition of B31-5A3/ bb0605 . Liquid chromatogram of mutanolysin treated PG isolated from B31-5A3 (top, in black) and B31-5A3/ bb0605 (bottom, in green). Each numbered peak corresponds to a muropeptide found in and , respectively. Peak 5 (shaded gray) corresponds to the muropeptide Glc N Ac-Mur N Ac-Ala-Glu-Orn-[Gly]-Ala-Ala). (D) MS (top) and MS/MS (bottom) of the Glc N Ac-Mur N Ac-Ala-Glu-Orn-[Gly]-Ala-Ala muropeptide. MS/MS fragmentation (inset) data confirms the pentapeptide(-Gly) structure.
Suprimescript Cdna Synthesis Kit, supplied by Genet Bio Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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toolsquant2 fast rt kit  (BioTools Co)
90
BioTools Co toolsquant2 fast rt kit
(A) Schematic of the putative bb0605 operon, as well as the downstream bb0608 locus, mapped from the B. burgdorferi B31 type strain genome. The transposon integration site (+300, 5’ to 3’) in bb0605 is shown (triangle) as are the length, and location, of predicted non-coding regions. (B) Relative mRNA levels of target loci to validate B31-5A3/ bb0605 <t>mutant.</t> <t>RT-PCR</t> was performed on RNA isolated from both parental (B31-5A3, charcoal) and mutant (B31-5A3/ bb0605, light gray) strains using locus-specific primers and normalized relative to constitutively expressed flaB. Values are the mean + /- SD. n.d., not detected after 45 cycles. (C) Identifying changes in the peptidoglycan composition of B31-5A3/ bb0605 . Liquid chromatogram of mutanolysin treated PG isolated from B31-5A3 (top, in black) and B31-5A3/ bb0605 (bottom, in green). Each numbered peak corresponds to a muropeptide found in and , respectively. Peak 5 (shaded gray) corresponds to the muropeptide Glc N Ac-Mur N Ac-Ala-Glu-Orn-[Gly]-Ala-Ala). (D) MS (top) and MS/MS (bottom) of the Glc N Ac-Mur N Ac-Ala-Glu-Orn-[Gly]-Ala-Ala muropeptide. MS/MS fragmentation (inset) data confirms the pentapeptide(-Gly) structure.
Toolsquant2 Fast Rt Kit, supplied by BioTools Co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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prime script rt kit (promega)  (Promega)
90
Promega prime script rt kit (promega)
(A) Schematic of the putative bb0605 operon, as well as the downstream bb0608 locus, mapped from the B. burgdorferi B31 type strain genome. The transposon integration site (+300, 5’ to 3’) in bb0605 is shown (triangle) as are the length, and location, of predicted non-coding regions. (B) Relative mRNA levels of target loci to validate B31-5A3/ bb0605 <t>mutant.</t> <t>RT-PCR</t> was performed on RNA isolated from both parental (B31-5A3, charcoal) and mutant (B31-5A3/ bb0605, light gray) strains using locus-specific primers and normalized relative to constitutively expressed flaB. Values are the mean + /- SD. n.d., not detected after 45 cycles. (C) Identifying changes in the peptidoglycan composition of B31-5A3/ bb0605 . Liquid chromatogram of mutanolysin treated PG isolated from B31-5A3 (top, in black) and B31-5A3/ bb0605 (bottom, in green). Each numbered peak corresponds to a muropeptide found in and , respectively. Peak 5 (shaded gray) corresponds to the muropeptide Glc N Ac-Mur N Ac-Ala-Glu-Orn-[Gly]-Ala-Ala). (D) MS (top) and MS/MS (bottom) of the Glc N Ac-Mur N Ac-Ala-Glu-Orn-[Gly]-Ala-Ala muropeptide. MS/MS fragmentation (inset) data confirms the pentapeptide(-Gly) structure.
Prime Script Rt Kit (Promega), supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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hiscript iii rt supermix (+gdna wiper)  (Novozymes limited)
90
Novozymes limited hiscript iii rt supermix (+gdna wiper)
(A) Schematic of the putative bb0605 operon, as well as the downstream bb0608 locus, mapped from the B. burgdorferi B31 type strain genome. The transposon integration site (+300, 5’ to 3’) in bb0605 is shown (triangle) as are the length, and location, of predicted non-coding regions. (B) Relative mRNA levels of target loci to validate B31-5A3/ bb0605 <t>mutant.</t> <t>RT-PCR</t> was performed on RNA isolated from both parental (B31-5A3, charcoal) and mutant (B31-5A3/ bb0605, light gray) strains using locus-specific primers and normalized relative to constitutively expressed flaB. Values are the mean + /- SD. n.d., not detected after 45 cycles. (C) Identifying changes in the peptidoglycan composition of B31-5A3/ bb0605 . Liquid chromatogram of mutanolysin treated PG isolated from B31-5A3 (top, in black) and B31-5A3/ bb0605 (bottom, in green). Each numbered peak corresponds to a muropeptide found in and , respectively. Peak 5 (shaded gray) corresponds to the muropeptide Glc N Ac-Mur N Ac-Ala-Glu-Orn-[Gly]-Ala-Ala). (D) MS (top) and MS/MS (bottom) of the Glc N Ac-Mur N Ac-Ala-Glu-Orn-[Gly]-Ala-Ala muropeptide. MS/MS fragmentation (inset) data confirms the pentapeptide(-Gly) structure.
Hiscript Iii Rt Supermix (+Gdna Wiper), supplied by Novozymes limited, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


(A) Schematic of the putative bb0605 operon, as well as the downstream bb0608 locus, mapped from the B. burgdorferi B31 type strain genome. The transposon integration site (+300, 5’ to 3’) in bb0605 is shown (triangle) as are the length, and location, of predicted non-coding regions. (B) Relative mRNA levels of target loci to validate B31-5A3/ bb0605 mutant. RT-PCR was performed on RNA isolated from both parental (B31-5A3, charcoal) and mutant (B31-5A3/ bb0605, light gray) strains using locus-specific primers and normalized relative to constitutively expressed flaB. Values are the mean + /- SD. n.d., not detected after 45 cycles. (C) Identifying changes in the peptidoglycan composition of B31-5A3/ bb0605 . Liquid chromatogram of mutanolysin treated PG isolated from B31-5A3 (top, in black) and B31-5A3/ bb0605 (bottom, in green). Each numbered peak corresponds to a muropeptide found in and , respectively. Peak 5 (shaded gray) corresponds to the muropeptide Glc N Ac-Mur N Ac-Ala-Glu-Orn-[Gly]-Ala-Ala). (D) MS (top) and MS/MS (bottom) of the Glc N Ac-Mur N Ac-Ala-Glu-Orn-[Gly]-Ala-Ala muropeptide. MS/MS fragmentation (inset) data confirms the pentapeptide(-Gly) structure.

Journal: PLOS Pathogens

Article Title: Peptidoglycan architecture dictates protein interactions, tissue tropism, and arthritis in the Lyme disease spirochete Borrelia burgdorferi

doi: 10.1371/journal.ppat.1013849

Figure Lengend Snippet: (A) Schematic of the putative bb0605 operon, as well as the downstream bb0608 locus, mapped from the B. burgdorferi B31 type strain genome. The transposon integration site (+300, 5’ to 3’) in bb0605 is shown (triangle) as are the length, and location, of predicted non-coding regions. (B) Relative mRNA levels of target loci to validate B31-5A3/ bb0605 mutant. RT-PCR was performed on RNA isolated from both parental (B31-5A3, charcoal) and mutant (B31-5A3/ bb0605, light gray) strains using locus-specific primers and normalized relative to constitutively expressed flaB. Values are the mean + /- SD. n.d., not detected after 45 cycles. (C) Identifying changes in the peptidoglycan composition of B31-5A3/ bb0605 . Liquid chromatogram of mutanolysin treated PG isolated from B31-5A3 (top, in black) and B31-5A3/ bb0605 (bottom, in green). Each numbered peak corresponds to a muropeptide found in and , respectively. Peak 5 (shaded gray) corresponds to the muropeptide Glc N Ac-Mur N Ac-Ala-Glu-Orn-[Gly]-Ala-Ala). (D) MS (top) and MS/MS (bottom) of the Glc N Ac-Mur N Ac-Ala-Glu-Orn-[Gly]-Ala-Ala muropeptide. MS/MS fragmentation (inset) data confirms the pentapeptide(-Gly) structure.

Article Snippet: All reactions were performed on the same plate, using PCRBIO 1-Step Go RT-PCR Kit (PCR Biosystems) following the recommended procedures.

Techniques: Mutagenesis, Reverse Transcription Polymerase Chain Reaction, Isolation, Tandem Mass Spectroscopy